Coding
Part:BBa_K925006:Design
Designed by: Michael Paul Lockhart Group: iGEM12_St_Andrews (2012-09-24)
My Precious
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 296
Illegal XhoI site found at 468 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 318
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When designing this part we based it around an existing protein, myoglobin, which had short α-helices and β-sheets. This allowed us to hijack the loops and turns of the myoglobin protein and inset our own metal binding sites. Some changes to the protein sequence was required as we used gBlocksTM which are a maximum of 500 base pairs in length.
Source
This part is biologically synthesised.