Coding

Part:BBa_K925006:Design

Designed by: Michael Paul Lockhart   Group: iGEM12_St_Andrews   (2012-09-24)


My Precious


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 296
    Illegal XhoI site found at 468
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 318
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing this part we based it around an existing protein, myoglobin, which had short α-helices and β-sheets. This allowed us to hijack the loops and turns of the myoglobin protein and inset our own metal binding sites. Some changes to the protein sequence was required as we used gBlocksTM which are a maximum of 500 base pairs in length.


Source

This part is biologically synthesised.

References